Abstract: This document presents the World Health Organization’s strategy for integrating gender analysis and actions into its work. It also reproduces resolution WHO60.25 which was adopted by the Sixtieth World Health Assembly in May 2007. The strategy elaborates four strategic directions that aim to enhance and expand WHO’s capacity to analyse the role of gender and sex in health and to monitor and address systemic and avoidable gender-based inequalities in health, as follows: building WHO capacity for gender analysis and planning; bringing gender into the mainstream of WHO’s management; promoting use of sex-disaggregated data and gender analysis; and establishing accountability. It is intended for all WHO managers, technical and administrative staff because gender is a cross-cutting issue.