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Author  |
Henke, Jura Inga; Goergen, Dagmar; Zheng, Yunfeng; Song, Yutong; Schüttler, Christian G.; Fehr, Carmen; Jünemann, Christiane; Niepmann, Michael |
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Title |
microRNA-122 stimulates translation of hepatitis C virus RNA |
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Journal Article |
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Year |
2008 |
Publication |
The EMBO Journal |
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Volume |
27 |
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Advance Online Publication 24; 20.11.2008 |
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1-11;3300 |
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Keywords |
hepatitis C; research |
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Abstract |
Hepatitis C virus (HCV) is a positive strand RNA virus that propagates primarily in the liver. We show here that the liver-specific microRNA-122 (miR-122), a member of a class of small cellular RNAs that mediate posttranscriptional gene regulation usually by repressing the translation of mRNAs through interaction with their 30-untranslated regions (UTRs), stimulates the translation of HCV. Sequestration of miR-122 in liver cell lines strongly reduces HCV translation, whereas addition of miR-122 stimulates HCV translation in liver cell lines as well as in the non-liver HeLa cells and in rabbit reticulocyte lysate. The stimulation is conferred by direct interaction of miR-122 with two target sites in the 50-UTR of the HCV genome. With a replication-defective NS5B polymerase mutant genome, we show that the translation stimulation is independent of viral RNA synthesis. miR-122 stimulates HCV translation by enhancing the association of ribosomes with the viral RNA at an early initiation stage. In conclusion, the liver-specific miR-122 may contribute to HCV liver tropism at the level of translation. |
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English |
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0261-4189 (Print); 1460-2075 (Electronic) |
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Call Number |
50-00831 |
Serial |
59668 |
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